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KMID : 1098420080160010020
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Korean Journal of Medicinal Crop Science
2008 Volume.16 No. 1 p.20 ~ p.26
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Phylogenetic Analysis of Ji-Mo (Anemarrhena asphodeloides) on the Basis of Chloroplast DNA Sequences
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Kim Myung-Kyum
Jigden Baigalmaa
Sun Hua
Noh Chong-Hoon
Kim Se-Young
Yang Deok-Chun
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Abstract
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Anemarrhena asphodeloides (Korean name "Ji-Mo") has been used for oriental medicinal purposes in Korea, China and Japan. In this study, 29 A. asphodeloides samples were collected including 3 certified A. asphodeloides plants and many commercially marketed A. asphodeloides products. Chloroplast trnL-F regions of the "Ji-Mo" samples were sequenced and used to identify whether the samples were genuine A. asphodeloides or not. As the result, the trnL-F sequences of all the "Ji-Mo" samples were shown to be identical and it was proven that commercially available medicinal products "Ji-Mo" are genuine A. asphodeloides. Phylogenetic tree of. A. asphodeloides using the trnL-F sequences was constructed and compared with phylogenetic tree using rubisco large subunit (rbcL) gene sequences. In these tree, A. asphodeloides was affiliated in the family Agavaceae in the order Asparagales. It is proven that trnL-F phylogenetic tree is useful to study taxonomic position of A. asphodeloides.
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KEYWORD
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Ji-Mo (Anemarrhena asphodeloides), Phylogenetic Analysis, rbcL, trnL-F
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